Smart Tube Thaw-Lyse Buffer, 1000X Concentrate, 2 Count

Thaw-Lyse Buffer, 1000X Concentrate is a lysis buffer used to lyse erythrocytes in samples that have been stored frozen in Smart Tubes or PROT1. The buffer is a 1000X concentrate that must be diluted 1:1000 with deionized water before use.

Thaw-Lyse Buffer contains a mixture of detergents and chaotropic agents that disrupt the cell membrane and lyse the erythrocytes. The buffer does not lyse leukocytes, platelets, or plasma proteins.

To use Thaw-Lyse Buffer, first thaw the frozen sample in a water bath or refrigerator. Then, add 1000 microliters of 1X Thaw-Lyse Buffer per 250 microliters of sample. Vortex the sample gently for 10 seconds. The erythrocytes will lyse, and the leukocytes and plasma proteins will remain intact.

Thaw-Lyse Buffer is a valuable tool for researchers who need to lyse erythrocytes in samples that have been stored frozen. It is a simple and effective way to prepare samples for downstream analysis, such as flow cytometry or microscopy.

Here are some of the key features of Thaw-Lyse Buffer, 1000X Concentrate:

• Lyzes erythrocytes in samples that have been stored frozen in Smart Tubes or PROT1
• A 1000X concentrate that must be diluted 1:1000 with deionized water before use
• Contains a mixture of detergents and chaotropic agents that disrupt the cell membrane and lyse the erythrocytes
• Does not lyse leukocytes, platelets, or plasma proteins
• Simple and effective way to prepare samples for downstream analysis

If you are a researcher who needs to lyse erythrocytes in samples that have been stored frozen, Thaw-Lyse Buffer, 1000X Concentrate is a valuable tool that can help you save time and improve your workflow.