SMART TUBE INC Lyse Buffer 2, 5X Concentrate, 500ml, 2 Count (1000ml)

SMART TUBE INC Lyse Buffer 2, 5X Concentrate 500ml is a high-quality lysis buffer that is used to lyse erythrocytes in whole blood samples that have been previously frozen in PROT1 or Smart Tubes. It is a concentrated buffer that must be diluted before use. The buffer contains a cocktail of enzymes that efficiently lyse erythrocytes without damaging DNA or RNA. It is compatible with a wide range of cell types and can be used for a variety of applications, including single-cell RNA sequencing, DNA microarrays, and protein expression analysis.

Here are some of the key features of SMART TUBE INC Lyse Buffer 2, 5X Concentrate 500ml:

• 5X concentrated buffer for convenient dilution
• Contains a cocktail of enzymes that efficiently lyse erythrocytes
• Compatible with a wide range of cell types
• Can be used for a variety of applications

Applications:

• Single-cell RNA sequencing
• DNA microarrays
• Protein expression analysis
• Other applications that require the lysis of erythrocytes

How to use:

1. Dilute the buffer 1:10 with water.
2. Add the diluted buffer to the whole blood sample.
3. Incubate the sample at room temperature for 5 minutes.
4. Centrifuge the sample at 1,000 RPM for 5 minutes.
5. The supernatant is the lysate, which can be used for downstream analysis.

Storage:

Store the buffer at -20°C.

Caution:

• Avoid contact with eyes and skin.
• If contact occurs, flush with water immediately.
• Keep out of reach of children.